Figure 3.

Time-dependent histological changes in skeletal muscle after CTX injection with balenine-enriched extract. (a) Sections (5 μm thick) of TA muscle from the normal diet and balenine-enriched extract diet groups were immunofluorescence stained with anti-laminin antibody, and stained with Hoechst33342. Red and blue indicate laminin and nuclei, respectively. Scale bar = 100 μm. Magnification is ×20. (b) The number of Hoechet33342-positive nuclei were counted in 13 high-power fields in three individual TA muscle sections and calculated by BZ-II analysis software. Data are means ± SD. Statistical analysis consisted of a one-way ANOVA and Tukey’s test. Different letters mean significant differences at p < 0.05. ND, mice fed the normal diet; Bal, mice fed the balenine-enriched extract diet.