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. 2022 May 5;20(5):313. doi: 10.3390/md20050313

Figure 6.

Figure 6

Effect of balenine on phagocytosis of RAW264.7 cells. (a) RAW264.7 cells were cultured with the indicated concentration of synthetic balenine for 24 h. Subsequently, 10 µg/mL lipopolysaccharide was treated with cells for 3 h. Cells were stained with 0.075% neutral red solution for 1 h, after which the optical density value of each well was measured at 570 nm, using a micro-plate reader. Cell phagocytic activity (%) was expressed by ODs/ODc × 100. ODs: OD values of the balenine wells. ODc: OD values of control wells. Data are means ± SD (n = 8). Statistical analysis consisted of a one-way ANOVA and Tukey’s test. ** p < 0.01, compared with the control group (0 mM balenine, PBS). (b) RAW264.7 cells were cultured with PBS or 10 mM balenine for 24 h. Opsonized 20 µg/mL E. coli BioParticales conjugated with Alexa Fluor 594 were treated in cells. One hour later, the number of beads in cells was counted in 12 high-power fields in 3 individual chambers, and expressed as means ± SD. * p < 0.05 compared with the control group (0 mM balenine, PBS). Con, control; Bal, synthetic balenine.