Figure 5.

Hypericum perforatum (HP1) and hypericin (HY) inhibit the growth of different SARS-CoV-2 variants. (A–F) Vero cells were seeded overnight, and the next day, before being infected, cells were incubated in infection-DMEM containing solvent control (DMSO) or either (A–C) HP1 or (D–F) hypericin for 1h, at 37 °C. Meanwhile, SARS-CoV-2 variants were also preincubated (1 h at room temperature) before infection in infection-PBS with either solvent control or (A–C) HP1 or (D–F) hypericin. After pre-incubation, virus infection was performed at a MOI of 0.05 for 1 h. After infection, cells were either incubated with solvent control or (A–C) HP1 or (D–F) hypericin. After 24 h infection, virus supernatants were collected, and virus titration was done by plaque assays. (A–F) Obtained data are shown as PFU/mL (mean and s.d.).