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. 2022 May 22;14(5):1112. doi: 10.3390/v14051112

Figure 1.

Figure 1

Evaluation of the A104R protein across ASFV isolates. (A) Amino acid alignment representing the diversity of A104R protein of ASFV in the field. Residues in white spots represent changes between amino acids with different charge. Conservation plot scores reflect the nature of the change in specific sites, with high scores associated with changes with similar biological properties. Alignment was produced using the software Jalview version 2.11.1.7. (B) Phylogenetic analysis conducted by maximum likelihood method and the Tamura-3 parameter model showing the diversity of A104R gene of ASFV in the field. Based on the cluster distribution, isolates were categorized in four main groups. Numbers above internal branches represent bootstrap values (1000 repetitions). (C) Comparison between the evolutionary rate of synonymous (dS) and nonsynonymous (dN) mutations during the evolution of A104R gene of ASFV. Significant differences between dS and dN rates were calculated by the unpaired T test. (D) The graphic represents the ratio dN-dS at specific codon sites in the A104R gene of ASFV. Red asterisks represent codon sites evolving under purifying selection. Analysis was conducted using the evolutionary algorithms FEL considering a cutoff value of p = 0.1.