Figure 1.

Schematic overview of the acute toxicity assay performed using coral larvae conducted in 12-well cell culture plates. The endpoint ‘mortality’ was assessed in the survival experiment, while ‘settlement’ (metamorphosis + attachment) was assessed additionally in the settlement assay. Planula larvae were exposed to the target compound for 48 h to assess acute toxic responses to the compound. A crustose coralline algae (CCA) chip was used to induce settlement. See Table S3 for more details on the exact condition during the experiments. ¹ Photo courtesy of L. Fiegel.