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. 2022 May 23;23(10):5859. doi: 10.3390/ijms23105859

Figure 1.

Figure 1

LAP2 is upregulated in sub-confluent rCEnCs (a). RT-PCR (Sybr Green) showing up and down-regulation of LAP2α and β across the passages in rCEnCs sub-confluent (S) and confluent (C) cultures. The LAP2 ability of being up and down-regulated decreases with passages (b). RT-PCR, divided between S and C populations highlighted how LAP2 expression decreases with passages till P6 when it is drastically reduced (c). Delta of LAP2 expression between S and C populations, studied across passages (P1 to P6) (d). RT-PCR of LAP2α and β expression in rCEnCs isolated from the Descemet tissues (T), compared with rCEnCs in culture at P0. Data are presented as mean ± SE. The statistical significance was determined by Students’ t-test. * p-value < 0.05. ** p < 0.01.