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. 2022 May 23;23(10):5859. doi: 10.3390/ijms23105859

Figure 2.

Figure 2

LAP2 protein increases in rCEnCs at S only at low passages. (a) Representative images of ki67 (red) and LAP2 (green) immunofluorescence staining of rCEnC at P2 S_C and P6 S_C. DAPI (blue) counterstained nuclei. (b) Representative images of αSMA (red) immunofluorescence staining of rCEnC at P2 S_C and P6 S_C. DAPI (blue) counterstained nuclei. (c) Quantification of immunofluorescence staining shown in (a,b). Data are presented as mean ± SE. The statistical significance was determined by Students’ t-test. * p-value < 0.05. ** p < 0.01. *** p < 0.001. (d) Western Blot analysis showing LAP2α expression on rCEnC P4 S_C. GAPDH was used as internal control.