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. 2022 May 23;23(10):5859. doi: 10.3390/ijms23105859

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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LAP2 expression in human CE (a) RT-PCR (Sybr Green) showing up and down-regulation of LAP2α and β in HCEnCs isolated from the tissues (T) and at subsequent passages (P0-P3) in S and C HCEnCs culture in vitro. (b) Representative images of LAP2 (red) immunofluorescence staining in cryosections of the human cornea, showing CE and stroma. DAPI (blue) counterstained nuclei. (c) Representative images of LAP2 (green) and ki67 (red) immunofluorescence staining on HCEnCs in vitro, at P1. DAPI (blue) counterstained nuclei. (d) Quantification of the immunofluorescence data shown in (c).