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. 2022 Apr 21;15(5):506. doi: 10.3390/ph15050506

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of G. alypum (GA), G. punctata (GP), G. cordifolia (GC) and G. meridionalis (GM) methanolic leaf extracts on oxidative stress biomarkers in Hep G2 cells cultured under hyperglycemic conditions: (a) glutathione S-transferase (GST) activity (U/mg protein); (b) glutathione peroxidase (GPx) activity (mU/mg protein); (c) free thiol groups (-SH) content (μmol/mg protein); (d) reduced glutathione (GSH) content (μmol/mg protein); (e) cell viability (%) assessed by the lactate dehydrogenase (LDH) assay; (f) cell viability (%) assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; Normal control: cells cultured in medium supplemented with 5.56 mM glucose; Hyperglycemia: cells cultured in medium supplemented with 20 mM glucose; # statistically significant difference in comparison to normal control (p < 0.05); * statistically significant difference in comparison to cells cultured in hyperglycemic conditions (p < 0.05); values are means + SD (n = 3; exceptionally, in the MTT assay, n = 8).