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. 2022 May 5;14(5):968. doi: 10.3390/v14050968

Figure 4.

Figure 4

ZIKV capsid expressing cells suppress IFN-β and are highly susceptible to infection. A549 cells were transduced with lentivirus expressing AcGFP or AcGFP-Myc-ZIKVC (MOT of 10). (A) AcGFP expressing cells have high levels of IFN-β mRNA. Cells were collected at 48 h post-transduction and the levels of IFN-β mRNA in mock, AcGFP or ZIKV C-expressing cells were determined by qRT-PCR. (B) ZIKV C expressing cells are permissive to ZIKV infection but are sensitive to pre-treatment with IFN-α. Transduced cells were infected with ZIKV at an MOI of 3 (grey bars) or pre-treated with IFN-α prior to ZIKV infection (pink bars). All cells were collected 48 h post-infection and stained with anti-ZIKV NS1 antibody. Only the GFP-positive cells were included in the analysis. Average results from 3 independent experiments are shown. Results of statistical analyses (t-test) are indicated (* p ≤ 0.05). ns, not significant.