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. 2022 May 5;14(5):968. doi: 10.3390/v14050968

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ZIKV capsid inhibits TRIM25 from ubiquitinating RIG-I. HEK293T cells were transfected with GST-tagged human RIG-I CARD domains (GST-h2CARD) or empty GST vector, together with HA-tagged ubiquitin (HA-Ub), V5-tagged human TRIM25 (hTRIM25-V5), and the indicated Flag-tagged proteins. Clarified whole cell lysates (WCL) were subjected to GST pulldown (GST PD), followed by immunoblotting (IB) with anti-GST, anti-HA, anti-V5, and anti-Flag antibodies. Influenza A virus (IAV) NS1 serves as a positive control for ubiquitination inhibition.