Table 2.
Primer pairs used for amplification of rickettsial genes.
| Gene | Primers | Primer Sequence (5′→3′) | Fragment Size (bp) | Tm (°C) | Reference |
|---|---|---|---|---|---|
|
gltA (nested) |
RpCS.877p | GGGGGCCTGCTCACGGCGG | 381 | 48 | [46,47] |
| RpCS.1258n | ATTGCAAAAAGTACAGTGAACA | ||||
| RpCS.896p | GGCTAATGAAGCAGTGATAA | 337 | 54 | ||
| RpCS.1233n | ATTGCAAAAAGTACAGTGAACA | ||||
|
ompA (semi nested) |
Rr190.70p | ATGGCGAATATTTCTCCAAAA | 631 | 46 | [46,48] |
| Rr190.701n | GTTCCGTTAATGGCAGCATCT | ||||
| Rr190.70p | ATGGCGAATATTTCTCCAAAA | 532 | 48 | ||
| Rr190.602n | AGTGCAGCATTCGCTCCCCCT | ||||
|
ompB (nested) |
rompB OF | GTAACCGGAAGTAATCGTTTCGTAA | 511 | 54 | [47] |
| rompB OR | GCTTTATAACCAGCTAAACCACC | ||||
| rompB SFG IF | GTTTAATACGTGCTGCTAACCAA | 420 | 56 | ||
| rompB SFG/TG IR | GGTTTGGCCCATATACCATAAG |