Table 3.
Bacteria | Target Gene | Primer Sequence (5′→3′) | Fragment Size (bp) |
Tm (°C) |
Reference |
---|---|---|---|---|---|
Bartonella spp. | rpoB | CGCATTGGCTTACTTCGTATG GTAGACTGATTAGAACGCTG | 825 | 53 | [49] |
Francisella tularensis | 17 KDa lipoprotein |
ATGGCGAGTGATACTGCTTG GCATCATCAGAGCCACCTAA |
250 | 56 | [50] |
Borrelia burgdorferi sensu lato |
Flagellin (nested) |
AARGAATTGGCAGTTCAATC GCATTTTCWATTTTAGCAAGTGATG |
497 | 52 | [51] |
ACATATTCAGATGCAGACAGAGGTTCTA GAAGGTGCTGTAGCAGGTGCTGGCTGT |
389 | 55 | [51,52] | ||
Coxiella burnetii | IS1111 | TATGTATCCACCGTAGCCAGTC CCCAACAACACCTCCTTATTC |
685 | 48 | [53] |
W: A/T; R: A/G.