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. 2022 Apr 30;11(5):528. doi: 10.3390/pathogens11050528

Table 3.

PCR primer pairs used for screening of Bartonella spp., Francisella tularensis, Borrelia burgdorferi sensu lato, and Coxiella burnetii.

Bacteria Target Gene Primer Sequence (5′→3′) Fragment
Size (bp)
Tm
(°C)
Reference
Bartonella spp. rpoB CGCATTGGCTTACTTCGTATG GTAGACTGATTAGAACGCTG 825 53 [49]
Francisella tularensis 17 KDa
lipoprotein
ATGGCGAGTGATACTGCTTG
GCATCATCAGAGCCACCTAA
250 56 [50]
Borrelia burgdorferi sensu lato Flagellin
(nested)
AARGAATTGGCAGTTCAATC
GCATTTTCWATTTTAGCAAGTGATG
497 52 [51]
ACATATTCAGATGCAGACAGAGGTTCTA
GAAGGTGCTGTAGCAGGTGCTGGCTGT
389 55 [51,52]
Coxiella burnetii IS1111 TATGTATCCACCGTAGCCAGTC
CCCAACAACACCTCCTTATTC
685 48 [53]

W: A/T; R: A/G.