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. 2022 Apr 23;11(5):503. doi: 10.3390/pathogens11050503

Table 2.

Moderating variables studied in the meta-analyses.

Moderating Variable Definition Classes *
Mosquito species Taxon to which the tested
individuals belong
  • Aedes caspius

  • Aedes detritus

  • Aedes vexans

  • Culex pipiens

  • Culex theileri

Viral titer of the blood meal Titer of virus in the blood on which the mosquitoes took their blood meal, i.e., host
viremia in the case of a live host or titer of virus in the artificial feeder
  • Low

  • Low to medium

  • Medium

  • Medium to high

  • High

Rearing generation of mosquitoes Field-collected or colonized lines of mosquitoes
  • F0/F1 (field-collected mosquitoes or first generation)

  • F2/F5 (colonized mosquitoes between second and fifth generation)

  • >F5 (mosquitoes over five generations of laboratory colonization)

Rearing temperature Temperature at which
mosquitoes were kept during
incubation
  • <20 °C

  • 20–25 °C

  • >25 °C

  • 13 then 26 °C

  • 20 then 28 °C

  • 22–26 °C

  • 26 °C (day)/22 °C (night)

Viral strain Viral strain used to infect mosquitoes
  • AN 1830

  • AnD133719

  • ArD141967

  • Clone 13

  • Kenya-128B-15

  • Lunyo

  • RVF MP-12

  • SH172805

  • strain 35/74

  • strain 56/74

  • T1

  • unknown (‘wild type’)

  • ZH501

  • ZH501 or Dak ArB 1976

  • ZH501 or Egypt93

  • ZH548

Time period between exposure and assays Time between infectious blood meal and assay for
mosquito infection (or dissemination or transmission)
Period known precisely
  • ≤1 week

  • >1 to ≤2 weeks

  • >2 to ≤3 weeks

  • >3 to ≤4 weeks

  • >4 weeks

Period provided as a range
  • <1 to ≤2 weeks

  • <1 to ≤3 weeks

  • <1 to ≤4 weeks

  • >1 to ≤3 weeks

  • >1 to ≤4 weeks

  • >1 to >4 weeks

  • >2 to 4 weeks

Country Country of origin of the
mosquito strain
  • Algeria

  • Canada

  • Cyprus

  • Egypt

  • UK

  • France

  • Germany

  • Lebanon

  • Morocco

  • Netherlands

  • Senegal

  • South Africa

  • Spain

  • Tunisia

  • USA

* See text for class definitions. Both viral strains are used in the same study without distinction.