Figure 7.
The virulence of M. tb positively modifies the expression and delivery of IL-1β. Human MDMs were obtained after seven days in culture. 2 × 106 MDMs were infected at MOI 1 and MOI 10 with an avirulent (H37Ra) and at MOI 1 y MOI 5 with a virulent (H37Rv) strain of M. tb 24 h postinfection cells were recovered and prepared for qPCR, and supernatant for ELISA. Relative gene expression of NLRP3 (a) and IL-1β (b) genes was assessed by qPCR and protein level of IL-1β by ELISA (c). Bar graphs show the mean ± SD from four or six independent experiments performed with two technical replicates. Statistical analysis was performed using Kruskal–Wallis analysis, followed by Dunn’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.