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. 2022 May 21;27(10):3313. doi: 10.3390/molecules27103313

Figure 6.

Figure 6

Effects of the methanol extract and isolated lignans 1–6 from M. sirikitiae leaves on LPS-induced mRNA expressions of inflammatory biomarkers in RAW 264.7 cells; Serum-starved cells were pretreated with crude methanol extract (C) (10 µg/mL) or lignans (5 or 10 µg/mL) for 3 h, and then stimulated with LPS for 6 h at 37 °C. After treatment, the total RNA was extracted from the cells and the mRNA expressions of TNF-α (A), IL-6 (B), IL-10 (C), NF-κB (D), COX-2 (E), and iNOS (F) were analyzed by RT-qPCR with gene-specific primers. The relative mRNA levels were quantified and shown as the mean ± SEM (n = 3). *, p < 0.05 vs. vehicle; #, p < 0.05 vs. LPS.