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. 2022 May 12;10(5):1016. doi: 10.3390/microorganisms10051016

Figure 2.

Figure 2

Twitching motility and type IV pilus (T4P) formation of A. citrulli M6 and an M6 mutant disrupted in gene APS58_1588 (M6-lip1-). Images of bacterial colonies of M6 (A) and M6-lip1- (B) seen by light microscopy after 48 h of growth on NA plates at 28 °C. Typical haloes surrounding the bulk colonies (indicated by the white double arrows) are formed by bacteria migrating via twitching motility. Pictures were taken using an Axio Scope light microscope equipped with a DXM1200F digital camera. Transmission electron microscopy observations of M6 (C) and M6-lip1- (D). The strains were observed in a FEI Tecnai-12 electron microscope after growth on NA at 28 °C for 48 h. Solid and dashed arrows indicate polar flagellum and T4P, respectively. Bars at the bottom of each panel: 100 µm for (A,B); and 0.5 µm for (C,D).