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. 2022 May 12;10(5):1016. doi: 10.3390/microorganisms10051016

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of swimming speed of A. citrulli M6, the M6-lip1^- mutant and the lip1 complemented mutant (M6-comp-lip1). Swimming speed measurements were calculated from NB-overnight cultures, as average of 150 bacterial cells for each strain, using a Nikon Eclipse T1-E inverted microscope equipped with a Digital Sight cooled monochrome CCD camera. Time-lapse pictures were taken and swimming speed was calculated using FIJI. Data represent averages and SE from one experiment out of three with similar results. Different letters indicate significant differences (p < 0.05) among treatments by Tukey–Kramer and ANOVA.