Figure 7.

Lipolytic activity of supernatants from A. citrulli M6, the M6-lip1^- mutant, and M6-lip1^- carrying plasmid pBBR-MCS-5 expressing lip1 (M6-comp-lip1). The strains were grown in XVM2 medium for 48 h at 28 °C with shacking (200 rpm). The supernatants were collected and used for measurement of lipolytic activity using 4-methylumbelliferyl oleate (MUO) as a substrate. Data represent averages and SE from three experiments with similar results (five replicates per strain in each experiment). Different letters indicate significant differences (p < 0.05) among treatments by Tukey–Kramer and ANOVA. MU, 4-methylumbelliferine; CFU, colony forming units.