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. 2022 May 12;10(5):1016. doi: 10.3390/microorganisms10051016

Figure 8.

Figure 8

Type II secretion (T2S) of Lip1. (A) Western blot analysis of extracellular and intracellular fractions of A. citrulli strains (AAC00-1, wild-type; AAC00-1-T2SS-, T2S mutant) expressing or not recombinant Lip1-HA (see details in Materials and Methods). Representative images of one out of two experiments with similar results are shown. Dashed lines between extracellular and intracellular proteins indicate that the images were composed for comprehensive visualization of treatments. The original images are shown in Figure S4. (B) Infiltration of four-week-old N. benthamiana leaves with strains M6, M6-lip1- and M6-lip1- expressing full length Lip1-HA or the Lip1 ORF lacking the N-terminal secretion signal (strains M6-comp-lip1-HA and M6-comp-lip135-383-HA, respectively). The picture was taken 36 h after infiltration and is representative of three independent experiments with similar results (with each experiment involving at least five leaves).