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. 2022 Apr 26;10(5):684. doi: 10.3390/vaccines10050684

Figure 1.

Figure 1

Design, expression, and characterization of rAb ZH9F7-Cap and isotype control 7DEU-Cap. (a) Schematic illustration of the recombinant mouse × pig chimeric rAb ZH9F7-Cap and isotype control rAb 7DEU-Cap. (b) 10% SDS–PAGE followed by Coomassie blue staining of purified rAb ZH9F7-Cap and rAb 7DEU-Cap. Lane 1: molecular weight marker; lane 2: rAb ZH9F7 without Cap; lane 3: purified rAb ZH9F7-Cap; lane 4: purified rAb 7DEU-Cap. (c) Western blot of the chimeric mouse × pig rAb ZH9F7-Cap and rAb 7DEU-Cap. Lane 1: molecular weight marker; lane 2: rAb ZH9F7 without Cap; lane 3: purified rAb ZH9F7-Cap; lane 4: purified rAb 7DEU-Cap. HRP-conjugated goat anti-porcine IgG (H + L) was used to confirm the expression of both mouse × pig recombinant antibodies.