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. 2022 Apr 26;10(5):684. doi: 10.3390/vaccines10050684

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Targeted skin DCs and lymphocytes migrate to regional inguinal nodes. (a) Strategy to analyze DEC205⁺-targeted cells in superficial inguinal lymph nodes. The CD3⁻ CD21⁻ population was analyzed for CD163 and CADM1 expression (bottom panel) and to identify potential DCs (CD3⁻ CD21⁻CADM1⁺ CD163⁻). (b) Analysis of rAb targeted DEC205⁺ cells from the control, rAb ZH9F7-Cap, and rAb 7DEU-Cap. (c) Average percentages of DCs and macrophages. PBS control (gray); rAb ZH9F7-Cap (blue); Isotype control rAb 7DEU-Cap (black). One-way ANOVA and the Tukey–Kramer test for multiple comparisons of means were performed (p < 0.05). p-values denote statistically significant differences in the means of the different groups.