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. 2022 Apr 26;14(5):936. doi: 10.3390/pharmaceutics14050936

Figure 2.

Figure 2

SMM-189 alters T cells and Th17 phenotypes during DSS-induced colitis. Spleens, MLNs, and LPLs were harvested from each of the three groups of C57BL6 mice described in the legend for Figure 1 and single-cell suspensions were prepared for each tissue. The cells were stained with antibodies specific for CD4, CD8, and IL-17 and analyzed by flow cytometry. Panel (A), the frequency in the lower right quadrant indicates the total percentage of CD4+ T cells and the upper left quadrant indicates the total percentage of CD8+ T cells. Panel (B), the frequency in the upper right quadrant indicates the total percentage of Th17 cells gated on CD4+ T cells. Panel (C), the percentage of total Th17 cells (n = 18; mean of six samples ± SEM) in the spleens, MLNs, and LPLs of each group was determined. Data are shown for a representative one of three independent experiments. Asterisks (*) (**) indicate a statistically significant difference at p < 0.01 and p < 0.001, respectively, between the group of mice treated with vehicle (DSS) and SMM-189.