Figure 6.

Induction of CB2, PKA, and histological alteration after SMM-189 treatment. LPLs and colon tissue were harvested from the three groups of mice in this study. (A) represents the immunoblot analysis of CB2 and PKA. (B) represents the relative fold expression of CB2 and (C) represents the relative fold expression of PKA. (D) representative histological sections of colons from the three groups of mice. DSS-treated mice that did not receive SMM-189 treatment exhibited significant lymphocyte infiltration and distortion of crypts (central panel arrow), but SMM-189-treated mice exhibited markedly decreased lymphocyte infiltration (right panel); these changes were not observed in tissue from mice in the control group (left panel). Other changes include diffuse leukocyte infiltrates and thickening of the lamina propria near the distorted crypts in the colon. (E) represents the combined inflammation scores, which were reduced for the SMM-189-treated group compared to DSS alone. Representative sections from three separate experiments (10X magnification) are shown, with each group containing six mice (n = 18). Asterisks (*) indicate statistically significant differences at p < 0.01 between the vehicle (DSS) and SMM-189-treated groups.