Table 4.

Stability results at the tested conditions (N = 3 samples per concentration level and time point).

Analyte	Concentration (ng/mL)		Bench Top (2 h, 25 °C)	Auto- Sampler (10 h, 10 °C)	1 FT Cycle (30 min, −18–25 °C)	2 FT Cycles (30 min, −18–25 °C)	3 FT Cycles (30 min, −18–25 °C)	Long-Term (2 Weeks, −18 °C)
RIB	62.5	Bias (%)	6.01	8.95	7.88	1.69	6.00	13.89
		CV (%)	5.07	9.50	12.89	11.16	5.02	14.77
	2500	Bias (%)	4.96	−6.47	11.10	−1.66	−4.69	−10.69
		CV (%)	1.69	5.18	7.05	7.05	1.61	4.45
ABE	37.5	Bias (%)	9.09	11.51	13.23	−7.43	14.07	7.60
		CV (%)	8.04	7.41	3.47	6.89	2.55	7.84
	1500	Bias (%)	−0.10	14.88	9.37	5.68	1.13	3.09
		CV (%)	3.82	4.25	1.56	1.56	6.93	5.21
PAL	6.2	Bias (%)	3.08	13.69	14.97	1.86	2.81	12.29
		CV (%)	10.27	1.35	4.99	6.62	4.84	9.95
	250	Bias (%)	−4.37	14.95	6.35	−1.65	−7.90	−3.08
		CV (%)	3.35	5.32	5.95	5.95	3.84	10.44
ANA	2.5	Bias (%)	−10.33	2.27	−7.88	−2.80	−3.98	3.09
		CV (%)	5.01	11.15	1.93	4.74	5.32	3.87
	100	Bias (%)	−0.62	4.89	6.65	8.39	−0.69	−0.40
		CV (%)	2.13	2.60	2.78	2.78	6.48	8.54
LET	6.25	Bias (%)	−6.81	6.11	−0.06	−6.52	−3.50	−10.44
		CV (%)	8.64	7.01	14.71	5.53	6.79	2.90
	250	Bias (%)	6.16	−0.29	2.07	−0.26	−7.61	−7.75
		CV (%)	1.66	2.95	2.80	2.80	9.51	8.45
FUL	12.5	Bias (%)	−9.36	7.38	−14.59	−13.24	−8.90	−5.01
		CV (%)	14.83	−3.68	10.68	14.99	4.62	9.00
	500	Bias (%)	2.32	−8.06	2.23	4.49	−7.51	−1.62
		CV (%)	1.74	1.37	8.71	8.71	7.33	4.46