Table 3.

Analytical conditions of the Ultra Performance Liquid Chromatography (UPLC).

Conditions	Parameters
Column	Agilent SB-C18 (1.8 µm, 2.1 mm × 100 mm)
Mobile phase	Mobile phase A (pure water with 0.1% formic acid) Mobile phase B (acetonitrile with 0.1% formic acid)
Gradient program	0 min	95:5 v/v (Mobile phase A: Mobile phase B)
	0–9 min	a linear gradient to 5:95 v/v
	9–10 min	5:95 v/v
	10–11.1 min	adjust to 95:5 v/v
	11.1–14 min	95:5 v/v
Flow rate	0.35 mL/min
Column temperature	40 °C
Injection volume	4 μL