Figure 2.

Effect of HT and its analogue on Ca²⁺-induced PS exposure in RBC. Cells were treated with Ca²⁺ ionophore in the presence of increasing concentrations of HT, Tyr and HA and PS exposure was evaluated as annexin-V-binding cells. (a) Original histogram of control cells (CTR). (b) Original histogram of Ca²⁺ ionophore-treated cells. (c) Histogram of phenols effect on Ca²⁺ ionophore treatment. Data are the means ± SD (n = 9). Statistical analysis was performed with one-way ANOVA followed by Tukey’s test. ### (p < 0.001) indicates a significant difference from CTR. ** (p < 0.01) and * (p < 0.05) indicates a significant difference from Ca²⁺ ionophore treatment. PS: phosphatidylserine; RBC: red blood cell, HT: hydroxytyrosol; Tyr: tyrosol; HA: homovanillic alcohol.