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. 2022 May 19;23(10):5693. doi: 10.3390/ijms23105693

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of HT and its analogue on Ca²⁺-induced MVs formation from RBC. Cells were treated with Ca²⁺ ionophore in the presence of increasing concentrations of HT, Tyr and HA and MVs formation was evaluated using FITC anti-human glycophorin A antibody. (a) Original histogram of control cells (CTR). (b) Original histogram of Ca²⁺ ionophore-treated cells. (c) Histogram of phenols effect on Ca²⁺ ionophore treatment. Data are the means ± SD (n = 9). Statistical analysis was performed with one-way ANOVA followed by Tukey’s test. ### (p < 0.001) indicates a significant difference from CTR. *** (p < 0.001) and * (p < 0.05) indicates a significant difference from Ca²⁺ ionophore treatment. MVs: microvesicles; RBC: red blood cell, HT: hydroxytyrosol; Tyr: tyrosol; HA: homovanillic alcohol; FITC: fluorescein isothiocyanate; DFC: dichlofluorescein.