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. 2022 May 19;23(10):5693. doi: 10.3390/ijms23105693

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of HT and its analogues on Ca²⁺-induced ROS production in RBC. Cells were treated with Ca²⁺ ionophore in the presence of increasing concentrations of HT, Tyr and HA and ROS production was evaluated by means of the fluorescent probe DCF. Data are the means ± SD (n = 9). Statistical analysis was performed with one-way ANOVA followed by Tukey’s test. ### (p < 0.001) indicates a significant difference from control (CTR). *** (p < 0.001), ** (p < 0.01), indicate a significant difference from Ca²⁺ ionophore treatment. ns (p > 0.05) indicates no significant difference from Ca²⁺ ionophore treatment. ROS: Reactive oxygen species; RBC: red blood cell; HT: hydroxytyrosol; Tyr: tyrosol; HA: homovanillic alcohol; DFC: dichlofluorescein.