Figure 6.

miR-195-5p mimic treatment reduces the intestinal inflammatory response in DSS-induced colitis. (A) Schematic diagram of DSS-induced colitis and miR-195-5p administration (n = 8 mice/group). After DSS treatment of mice, body weight loss (B) and disease activity index (C) were assessed during treatment in each group. (D) miR-195-5p expression levels by Real-time PCR in all colon portions (proximal, medial and distal) of treated mice compared to vehicle mice. (E) Cldn2 expression levels by Real-time PCR in all colon portions (proximal, medial and distal) of treated mice compared to vehicle mice. (F) Representative images of hematoxylin and eosin staining of distal colon tissue from each group taken at 10× magnification. Scale bar 10 μm. (G) Histological score of treated and control mice in all colon portions (proximal, medial and distal). * p < 0.05, ** p < 0.001.