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. 2022 Apr 21;14(5):911. doi: 10.3390/pharmaceutics14050911

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Dose–concentration curves for ESO and ESON inhibition on pore formation activity. (A) No treated cells and cells treated with ATP analyzed with PI-uptake assay assay; (B) The graph represents the quantification of dye uptake assays performed to 37 °C on MPM. Dose-response curves of ESO and ESON with or without 1 mM ATP for 25 min analyzed by fluorescence plate reader to detect the PI uptake; (C) No treated cells and cells treated with ATP analyzed with LY-uptake assay; (D) dose–response curves of ESO and ESON with or without 1 mM ATP for 25 min analyzed by fluorescence plate reader to detect the LY uptake. Values represent mean + SEM. Profiles are representative of 3–6 independent experiments.