Table 1.

HPLC-ESI-MS/MS tentative identification of the metabolites present in the methanol and dichloromethane extracts of the aerial parts of sea heath (F. laevis).

No.	Name	Formula	Rt	[M + H]+	[M − H]−	Methanol	Dichloromethane
1 1	Citric acid	C6H8O7	2.57		191.01918	++	+
2 1	Gallic acid (3,4,5-Trihydroxybenzoic acid)	C7H6O5	3.25		169.01370	+	-
3	Gallic acid sulfate	C7H6O8S	3.55		248.97051	+	-
4 2	3-O-Methylgallic acid-5-O-sulfate	C8H8O8S	8.69		262.98616	+	-
5	Uralenneoside	C12H14O8	11.09		285.06105	+	-
6	(Trihydroxyphenyl)propanoic acid hexoside-O-sulfate	C15H20O13S	11.80		439.05464	+	-
7	Benzylhexose sulfate	C13H18O9S	11.95		349.05933	+	-
8	Dihydroxy-methoxybenzoic acid	C8H8O5	12.13		183.02935	+	-
9	Feruloylhexose sulfate	C16H20O12S	12.50		435.05972	+	-
10	Caffeoylhexose sulfate	C15H18O12S	12.79		421.04408	+	-
11	Caffeic acid sulfate	C9H8O7S	13.89		258.99125	+	-
12	Coumaroylhexose sulfate	C15H18O11S	13.92		405.04916	+	-
13	p-Coumaric acid 4-O-sulfate	C9H8O6S	14.26		242.99634	+	-
14	Unidentified sulfate (Vanillin derivative)	C20H22O9S	15.11		437.09063	+	-
15	Butenylpyrocatechol sulfate or isomer	C10H12O5S	15.54		243.03272	++	+
16	Phenylethylhexose sulfate	C14H20O9S	15.71		363.07498	++	+
17	12-Hydroxyjasmonic acid sulfate or Tuberonic acid sulfate	C12H18O7S	16.19		305.06950	+	-
18	Caffeoylpentose sulfate	C14H16O11S	16.40		391.03351	+	-
19	Ferulic acid 4-O-sulfate	C10H10O7S	16.51		273.00690	+	-
20	Butanoylpyrocatechol sulfate or isomer	C10H14O5S	16.69		245.04837	++	+
21	Lyoniresinol sulfate	C22H28O11S	17.59		499.12741	+	-
22	Lariciresinol or isomer sulfate	C20H24O9S	18.21		439.10628	+	-
23	Isololiolide	C11H16O3	18.54	197.11777		+	++
24	Loliolide	C11H16O3	19.75	197.11777		+	++
25	3-O-Methylellagic acid-4′-O-glucoside	C21H18O13	21.30		477.06692	+	-
26	3,3′-Di-O-methylellagic acid-4-O-glucoside	C22H20O13	21.76		491.08257	+	-
27	Isorhamnetin-O-pentosylhexoside	C27H30O16	21.99		609.14556	+	-
28	N-cis-Feruloyltyramine	C18H19NO4	22.28	314.13924		+	++
29	Ellagic acid	C14H6O8	22.79		300.99845	+	-
30	3-O-Methylellagic acid-4-O-sulfate	C15H8O11S	24.10		394.97091	+	-
31	Kaempferol sulfate isomer 1	C15H10O9S	24.53		364.99673	+	-
32	N-trans-Feruloyltyramine	C18H19NO4	24.55	314.13924		+	++
33	3,3′-Di-O-methylellagic acid-4-O-sulfate	C16H10O11S	24.82		408.98656	+	-
34	3-O-Methylellagic acid	C15H8O8	25.19		315.01410	+	-
35	N1,N5,N10-Tricoumaroylspermidine isomer 1	C34H37N3O6	26.22		582.26042	++	+
36	Dihydroactinidiolide	C11H16O2	26.58	181.12286		+	++
37	Kaempferol sulfate isomer 2	C15H10O9S	26.60		364.99673	+	-
38	3,3′,4-Tri-O-methylellagic acid-4′-O-sulfate	C17H12O11S	26.99		423.00221	++	+
39	N1,N5,N10-Tricoumaroylspermidine isomer 2	C34H37N3O6	27.25		582.26042	+	-
40	3,3′-Di-O-methylellagic acid	C16H10O8	27.45		329.02975	+	++
41	N1,N5,N10-Tricoumaroylspermidine isomer 3	C34H37N3O6	28.10		582.26042	+	-
42	N1,N5,N10-Tricoumaroylspermidine isomer 4	C34H37N3O6	28.86		582.26042	+	-
43	3,3′,4-Tri-O-methylellagic acid	C17H12O8	29.90		343.04540	+	-
44	Malyngic acid	C18H32O5	32.28		327.21715	+	++
45	Hydroxyoctadecatrienoic acid isomer 1	C18H30O3	39.73		293.21167	-	+
46	Hydroxyoctadecatrienoic acid isomer 2	C18H30O3	39.95		293.21167	-	+
47	Oxophytodienoic acid	C18H28O3	40.00		291.19603	-	+
48	Hexadecanedioic acid	C16H30O4	40.53		285.20659	-	+
49	Hydroxyoctadecatrienoic acid isomer 3	C18H30O3	41.11		293.21167	-	+
50	Hydroxyoctadecadienoic acid	C18H32O3	41.16		295.22732	-	+
51	Pheophytin A	C55H74N4O5	62.57	871.57375		+	++

HPLC-ESI-MS/MS: high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. Rt: retention time. +: Presence; ++: Presence in higher abundance; -: Absence. ¹ Confirmed by standard. ² Hussein [15].