Table 2.
Antioxidant activities of the methanol and dichloromethane extracts of sea heath’s (F. laevis) aboveground biomass. The results are expressed as half-maximal effective concentration (EC50) values (mg/mL).
| Assay | Methanol | Dichloromethane | Positive Control |
|---|---|---|---|
| RSA-DPPH | 0.25 ± 0.01 b | >1 mg/mL | 0.11 ± 0.00 a |
| RSA-ABTS | 0.65 ± 0.02 b | >1 mg/mL | 0.06 ± 0.00 a |
| FRAP | 0.51 ± 0.03 | >1 mg/mL | na |
| CCA | 0.78 ± 0.01 b | >1 mg/mL | 0.17 ± 0.00 a |
| ICA | >1 mg/mL | 0.76 ± 0.05 b | 0.06 ± 0.00 a |
Values represent the mean ± standard error of the mean (SEM) of at least three experiments performed in triplicate (n = 9). In the same line, the values followed by different letters (a, b) are significantly different at p < 0.05 (Student’s t-test). RSA–DPPH: radical scavenging activity on 2,2-diphenyl-1-picrylhydrazyl; RSA–ABTS: radical scavenging activity on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid; FRAP: ferric reducing antioxidant power; CCA: copper chelating activity; ICA: iron chelating activity; na: not applicable. Positive controls: butylated hydroxytoluene (BHT, E320) for DPPH, ABTS and FRAP, and ethylenediamine tetraacetic acid (EDTA) for CCA and ICA.