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. 2022 May 23;23(10):5857. doi: 10.3390/ijms23105857

Figure 2.

Figure 2

Selective expansion of NKG2C and NKG2A NK cell subsets with feeder cells. (a) Flow cytometry analysis of NKG2A and NKG2C expression at day 0 in CD56+/CD3− NK cells from one representative donor followed by expansion using the depicted feeder cell lines and analysis of NKG2A and NGK2C NK cell subsets at day 14. (b) After 14 days of expansion, NK cells were counted, and the expansion factor (x-fold of starting number of NK cells) was calculated (n = 6). (c) Analysis of NKG2+ NK cell subsets in the differentially expanded NK cells and in corresponding fresh NK cells from same donors (n = 6). PC3PSCA-IL-2-mIL-15d-HLA-E*spG feeder cells promote selective outgrowth and therefore a higher frequency of NKG2C single-positive NK cells when compared to fresh NK cells and NK cells expanded with PC3PSCA-IL-2-mIL-15d feeder cells. Vice versa, PC3PSCA-IL-2-mIL-15d feeder cells preferentially promote the generation of NKG2A single-positive NK cells. Dots and the horizontal lines inside the Whisker boxes indicate the mean and the median. ** p < 0.01, *** p < 0.001.