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. 2022 May 23;23(10):5857. doi: 10.3390/ijms23105857

Figure 7.

Figure 7

Ectopic expression of HLA-E*spG significantly increases susceptibility of HLA/KIR-matched GBM target cells towards NKG2C+ NK cell-mediated lysis. (a) Flow cytometry analysis of HLA class I as well as of HLA-G, and HLA-E/HLA-E*spG surface expression in parental HT18584 and HT18584-HLA-E*spG glioblastoma cells. Isotype staining controls are included (grey histograms). As depicted in the accompanying table, the transgenic expression of HLA-E*spG results in an increased MFI for HLA-E but expression levels for HLA class I and HLA-G remain mostly unchanged. (b) Chromium release assay demonstrating increased killing of HT18584-HLA-E*spG target cells by sorted NKG2C+ NK cells when compared to parental HT18584 cells. * p < 0.05, ** p < 0.01.