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. 2022 May 23;23(10):5833. doi: 10.3390/ijms23105833

Figure 2.

Figure 2

WISH and IF of thyroid function. (A–J’) WISH of HPT axis markers in BaP treated embryos (50 nM) and controls (1.2 % DMSO) at 54 and 80 hpf. (A–B’ and C–D’) tg and nis at the level of thyroid tissue; (E–F’) trh expression in the hypothalamic region; (G–H’ and I–J’) tshba and dio2 in the anterior pituitary. Each experiment was performed in triplicate using about 30 embryos/stage. Embryos were acquired in dorsal (A–D’) or ventral (E–J’) views, anterior to the left. (K,K’) IF of T4 produced in thyroid follicles of DMSO and BaP treated larvae at 120 hpf. Thyroid regions were acquired mounting the heads in ventral view, anterior to the left. (L) Number of T4-positive follicles. Results are expressed by mean ± SD (*** p < 0.001).