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. 2022 May 28;12:8983. doi: 10.1038/s41598-022-12995-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Enhanced expression of AXL is closely associated with OR1 and OR2 cell growth. (a) Protein expression levels of AXL in H1975, OR1 and OR2 cells by western blot analysis. (b) AXL protein expression levels in three cell lines treated with control or AXL siRNA for 3 days. (c) Effects of AXL silencing on growth of three cell lines. Each value is the mean ± SD of triplicate dishes. *P < 0.05, **P < 0.01, two-tailed Student’s t-test. (d) Effects of AXL silencing on expression of pSFK, pAKT, and pERK in the three cell lines. Cell were treated with an AXL siRNA for 3 days followed by western blot analysis. (e) Combination of osimertinib and AXL siRNA on OR1 and OR2 cell growth. Cells were exposed to 0.4 μM osimertinib and/or AXL siRNA for 3 days. Each value is the mean ± SD of triplicate dishes. *P < 0.05, **P < 0.01. (f) Expression levels of pSFK, pAKT, and pERK were analyzed after treatment with various doses of R428 for 2 days. (g) Sensitivity to osimertinib with or without R428. Cells were exposed for 3 days and subjected to a water-soluble tetrazolium salt (WST) assay. Each value is the mean ± SD of triplicate dishes.