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. 2022 May 28;12:8984. doi: 10.1038/s41598-022-12736-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of interaction between promotor F44E5.4 and Hsf-1 DBD via titration in SV-AUC. HSF-1 DBD was titrated to the promoter F44E5.4 in concentrations ranging from a 0–15-fold excess. A shift to the right represents DNA-binding by the HSF-1 DBD. (a) dc/dt plot of the absorbance measured at 260 nm and for (b) at 280 nm at different concentrations of HSF-1 DBD, when added to 1.5 µM of HSE-containing DNA.