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. 2022 May 28;13(5):506. doi: 10.1038/s41419-022-04908-4

Fig. 2. Exosomes derived from RBP-J overexpressed macrophages inhibit proliferation and invasion of glioma cells.

Fig. 2

A Exosomes isolated from WT THP-1 derived macrophages (WT Mφ-Exo) and RBP-J-overexpressed macrophages (RBP-J OE Mφ-Exo) imaged by transmission electron microscopy (TEM) and their size distribution were measured using NTA. Scale bar = 50 nm. B Levels of exosome markers CD63 and TSG101 in WT or RBP-J OE Mφ-Exo were determined by Western blotting. C WT Mφ-Exo or RBP-J OE Mφ-Exo were labeled with PKH67; green represents PKH67, and blue represents nuclear DNA staining by DAPI. U87 MG cells were incubated with WT Mφ-Exo or RBP-J OE Mφ-Exo for 3 h. D, E Cell proliferation in glioma cell lines U87 MG and U373 MG treated with WT Mφ-Exo or RBP-J OE Mφ-Exo was assessed by CCK-8 (D) and EdU assay (E). F Transwell invasion assay is performed to indicate cell invasion (bar = 100 μm). All experiments were performed three times. *P < 0.05 and **P < 0.01 for statistical differences.