Table 2.
Sequences of primers for qRT-PCR and siRNA related sequence.
| Name | Sequence | |
|---|---|---|
| circRNA BTG2 | Forward | 5’- TGGAAGAATGTACAGCTTATGGA-3’ |
| Reverse | 5’- CTCGGGCTCAGTGAGAGGT-3’ | |
| PTEN | Forward | 5’- TGGATTCGACTTAGACTTGACCT-3’ |
| Reverse | 5’- GGTGGGTTATGGTCTTCAAAAGG-3’ | |
| GAPDH | Forward | 5’-GGCTGTTGTCATACTTCTCATGG-3’ |
| Reverse | 5’-GGATCTCGCTCCTGGAAGATG-3’ | |
| U6 | Forward | 5’-CTCGCTTCGGCAGCACA-3’ |
| Reverse | 5’-AACGCTTCACGAATTTGCGT-3’ | |
| miR-25-3p | Forward | 5’- ACACTCCAGCTGGGCAUUGCACUUGUCUCG-3’ |
| Reverse | 5’- CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCGAGACAA-3’ | |
| circRNA BTG2 siRNA | Forward | 5’- GUAGGAUAACAGGGUAACGCUUU-3’ |
| Reverse | 5’- AGCGUUACCCUGUUAUCCUACUU-3’ | |
| miR-25-3p mimics | Sense | 5’- CAUUGCACUUGUCUCGGUCUGA-3’ |
| Antisense | 5’- AGACCGAGACAAGUGCAAUGUU-3’ | |
| miR-25-3p inhibitor | Sense | 5’- UCAGACCGAGACAAGUGCAAUG-3’ |