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. Author manuscript; available in PMC: 2022 May 29.
Published in final edited form as: Chem Rev. 2021 Aug 18;121(22):13701–13796. doi: 10.1021/acs.chemrev.1c00308

Figure 13.

Figure 13.

Metal Active Sites by Covalent Tethering (MASCoT). a) Implementation of MASCoT. The C96-C96 disulfide bond covalently tethers two proteins, forming a nascent protein-protein interface that is locked into place upon metal addition. b-e) Formation of metal binding sites with different primary and secondary coordination spheres. Adapted with permission from Ref.347. Copyright 2019 NPG.