Figure 13.
Metal Active Sites by Covalent Tethering (MASCoT). a) Implementation of MASCoT. The C96-C96 disulfide bond covalently tethers two proteins, forming a nascent protein-protein interface that is locked into place upon metal addition. b-e) Formation of metal binding sites with different primary and secondary coordination spheres. Adapted with permission from Ref.347. Copyright 2019 NPG.