Figure 20.
Oligomerization of oppositely supercharged Cerulean (Ceru) and GFP variants mediated by electrostatic interactions. a) Hypothesized model of symmetric oligomers and long-range assembly of oppositely supercharged monomeric proteins. b) Supercharged GFP variants engineered by mutating the surface residues of sfGFP to basic (blue) or acidic (yellow) residues. c) Cryo-EM reconstruction of the Ceru+32/GFP−17 16-mer at 3.47 Å resolution in three different orientations. Adapted with permission from Ref.276. Copyright 2019 NPG.