Figure 23.

MDPSA and MeTIR strategies for metal-mediated oligomerization. In MDPSA, metal-chelating motifs are installed on the surface of target proteins, thereby guiding protein self-assembly through metal-coordination. In MeTIR strategy, metal-directed protein assemblies are redesigned using non-covalent interactions and a covalent linker at the protein-protein interface. The sequential design approach through MDPSA and MeTIR results in the formation of stable oligomers without the aid of metal ions. Adapted with permission from Ref. 83. Copyright 2016 Elsevier.