Figure 24.

Metal-mediated protein oligomers based on cyt cb₅₆₂ as a building block. a) Design of cyt cb₅₆₂ oligomers using the MDPSA approach. In the MBPC2 variant, surface-exposed bis-His clamps are located at residues 59/63 and 73/77. Depending on the metal ion coordination preferences, different oligomerization states are achieved – Ni₂:MBPC1₃ trimer (top), Cu₂:MBPC1₂ dimer (middle), and Zn₄:MBPC1₄ tetramer (bottom). b) In the MBPC2 variants, the R62D and D74A mutations give rise to the Zn₄:MBPC2₄ tetramer where the orientation of the protein monomers is inverted. c) MeTIR strategy for redesigning the interface of Zn₄:MBPC1₄. i1 interface of Zn₄:MBPC1₄ tetramer is redesigned to install hydrophobic residues and create the stabilized Zn₄:RIDC1₄ tetramer. (b) Adapted with permission from Ref.³⁶⁶. Copyright 2008 ACS. (c) Adapted with permission from Ref.⁸⁸. Copyright 2019 ACS.