Figure 29.
Design scheme of protein cages by genetic fusion using oligomers and coiled-coil domains. a) Trimeric KDGP-aldolase building blocks connected with antiparallel coiled-coil domains self-assemble into heterogeneous structures. b) Different combinations of symmetry elements are obtained by fusing the C-terminus of a trimeric protein to coiled-coil-forming peptides with different oligomerization states. Protein cages of different geometry are observed by ns-TEM or cryo-EM. (a-b) Adapted with permission from Ref.141. Copyright 2017 Wiley. (b) Adapted with permission from Ref.140. Copyright 2016 National Academy of Science. Adapted with permission from Ref.142. Copyright 2019 ACS.