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. Author manuscript; available in PMC: 2022 May 29.
Published in final edited form as: Chem Rev. 2021 Aug 18;121(22):13701–13796. doi: 10.1021/acs.chemrev.1c00308

Figure 75.

Figure 75.

Thermal and chemical stability of designed protein assemblies and their components. a) Denaturation of MIC1 in various assembly states (monomer, Cu2+-mediated cage, cage + EDTA, cage + EDTA + Cu2+ (rescued assembly)) induced by GuHCl titration (left) or thermal denaturation (right) monitored by CD spectroscopy at 222 nm. b) Stability of monomeric and self-assembled RIDC3 in THF and iPrOH. c) Thermal and pH-dependent stability of TRAP cages tracked by native PAGE and ns-TEM. d) Thermally induced unfolding of the Ico8 protein cage and the TriEst subunit monitored by changes in molar ellipticity at 222 nm (left) and by light scattering (right). (a) Adapted with permission from Ref.401. Copyright 2013 NPG. (b) Adapted with permission from Ref.204. Copyright 2014 National Academy of Sciences. (c) Adapted with permission from Ref.200. Copyright 2019 NPG. (d) Adapted with permission from Ref.142. Copyright 2019 ACS.