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. 2022 May 28;20:245. doi: 10.1186/s12951-022-01458-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — NIR laser irradiation of pTRG-treated tumors induced the activation of conventional dendritic cells (cDCs) in tumor-draining lymph nodes (tdLNs). A Schematic illustration of the analysis method for DC activation in tdLNs. B cDC subset analysis in tdLNs via flow cytometry. C The mean fluorescence intensities (MFIs) of CD40, CD80, CD86, MHC I, and MHC II in cDC1 (upper panel) and cDC2 (lower panel) were measured via flow cytometry (n = 6, two-way ANOVA, mean ± SEM, *p < 0.05, **p < 0.01). D Serum concentrations of indicated cytokines were analyzed using enzyme-linked immunosorbent assays (ELISAs) (n = 6, two-way ANOVA, mean ± SEM, **p < 0.01)