Table 5.
Experimental design matrix describing illudin M production in response to medium components
| Trial | Factor 1 (CSS g L−1) | Factor 2 (glucose g L−1) | Response | |||
|---|---|---|---|---|---|---|
| illudin M (mg L−1) | ||||||
| Actual | Coded | Actual | Coded | Actual | Predicted | |
| 1 | 3 | − 1 | 9 | − 0.5 | 183 | 185 |
| 2 | 3 | − 1 | 12 | 0 | 207 | 204 |
| 3 | 3 | − 1 | 15 | 0.5 | 192 | 193 |
| 4 | 6 | 0 | 6 | − 1 | 265 | 266 |
| 5 | 6 | 0 | 9 | − 0.5 | 318 | 322 |
| 6 | 6 | 0 | 12 | 0 | 365 | 349 |
| 7 | 6 | 0 | 15 | 0.5 | 333 | 345 |
| 8 | 6 | 0 | 18 | 1 | 315 | 312 |
| 9 | 9 | 1 | 9 | − 0.5 | 272 | 264 |
| 10 | 9 | 1 | 12 | 0 | 283 | 298 |
| 11 | 9 | 1 | 15 | 0.5 | 310 | 302 |
The cultures were prepared according to method SP4. The highest titer was measured at the central point of the experimental space G12/C6