Fig. 2.

Synergy between loss of Gpr124 and loss of Wnt7a in the severity of limb development phenotypes. (A) Alcian Blue staining of the skeleton at E15 showing progressively more severe truncations with progressively reduced Wnt7a and/or Gpr124 function (from left to right). Insets, images of the foot rotated to more clearly show the digits. (B) Quantification of E15 limb skeletal defects with progressively reduced Wnt7a and/or Gpr124 function (from left-to-right). The scoring procedure is described in the Materials and Methods section. P-values were calculated using the non-parametric Mann–Whitney–Wilcoxon test. Error bars show the mean±s.d. Each circle represents a single limb.