Fig. 3.

Synergy between Reck and Wnt7a loss in the severity of limb development phenotypes. (A) Alcian Blue staining of the skeleton at E15 showing that, in a Reck^Δex2/Δex2 background, reducing the number of WT copies of Wnt7a from one to zero increases the severity of fore and hind limb skeletal defects. Red arrows point to regions with local bleeding (see Fig. S1 for additional examples). (B) Quantification of E15 limb skeletal defects with progressively reduced Wnt7a and/or Reck function (from left to right). Δ, Reck^Δex2; PW, Reck^PW. The scoring procedure is described in the Materials and Methods section. P-values were calculated using the non-parametric Mann–Whitney–Wilcoxon test. Error bars show the mean±s.d. Each circle represents a single limb.